Plant Degradomics
Plants contain hundreds of proteases (e.g. >800 in Arabidopsis thaliana) that have been implicated in virtually all aspects of plant biology. However, few specific protease-substrate relationships have been resolved. What are their substrates? Where, when and under which conditions are substrates cleaved or degraded? What is the fate and function of cleaved substrates?
We use 'degradomics' to address these questions on a systems scale.
Further reading:
Quantitative proteomics in plant protease substrate identification. New Phytol 218(3):936-943.
We use 'degradomics' to address these questions on a systems scale.
Further reading:
Quantitative proteomics in plant protease substrate identification. New Phytol 218(3):936-943.
Proteolysis in plant microbe-interactions
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Proteases and protease inhibitors direct the outcome of plant-microbe interactions. Plant proteases contribute to immune signaling, regulated cell death and are molecular weapons against invading microbes, whereas microbes secrete proteases and protease inhibitors to avoid recognition, suppress host defense mechanisms and manipulate host metabolism.
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To understand the underlying molecular mechanisms, we investigate:
- Which proteins are cut as part of immune signaling cascades?
- What are the substrates of plant defense and microbial effector proteases?
- How is pathogen-triggered cell death initiated and executed?
- Which proteins are cut as part of immune signaling cascades?
- What are the substrates of plant defense and microbial effector proteases?
- How is pathogen-triggered cell death initiated and executed?
Recent collaborative work with the Döhlemann lab in Cologne:
Combination of transcriptomic, proteomic, and degradomic profiling reveals common and distinct patterns of pathogen-induced cell death in maize. Plant J. 116(2):574-596.
A fungal substrate mimicking molecule suppresses plant immunity via an inter-kingdom conserved motif. Nat Commun. 10(1):1576.
Dual function of a secreted fungalysin metalloprotease in Ustilago maydis. New Phytol 220:249-61.
Combination of transcriptomic, proteomic, and degradomic profiling reveals common and distinct patterns of pathogen-induced cell death in maize. Plant J. 116(2):574-596.
A fungal substrate mimicking molecule suppresses plant immunity via an inter-kingdom conserved motif. Nat Commun. 10(1):1576.
Dual function of a secreted fungalysin metalloprotease in Ustilago maydis. New Phytol 220:249-61.
Proteolysis in plant abiotic stress responses
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Plants can cope with strong changes in environmental conditions, including tremendous fluctuations light intensity and temperature on a different time scales. We are investigating proteolytic mechanisms that contribute to these responses with a focus on proteostasis and proteolytic signaling in chloroplasts and mitochondria.
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Further reading:
A phloem-localized Arabidopsis metacaspase (AtMC3) improves drought tolerance. New Phytol. 239(4):1281-1299.
Abundance of metalloprotease FtsH12 modulates chloroplast development in Arabidopsis thaliana. J Exp Bot 72(9):3455-73.
DEG10 contributes to mitochondrial proteostasis, root growth, and seed yield in Arabidopsis. J Exp Bot 70(19):5423-36.
A phloem-localized Arabidopsis metacaspase (AtMC3) improves drought tolerance. New Phytol. 239(4):1281-1299.
Abundance of metalloprotease FtsH12 modulates chloroplast development in Arabidopsis thaliana. J Exp Bot 72(9):3455-73.
DEG10 contributes to mitochondrial proteostasis, root growth, and seed yield in Arabidopsis. J Exp Bot 70(19):5423-36.
We thank the following agencies and programs for supporting plant degradomics :
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