Publications by team members. * corresponding author, # equal contribution.

2020

  1. Hofsetz E, Demir F, Szczepanowska K, Kukat A, Kizhakkedathu J, Trifunovic A*, Huesgen PF*. The mouse heart mitochondria N terminome provides insights into ClpXP-mediated proteolysis. Mol Cell Proteomics. 2020 May 28:mcp.RA120.002082. doi: 10.1074/mcp.RA120.002082. Online ahead of print.
  2. Niedermaier S, Schneider T, Bahl M-O, Matsubara S*, Huesgen PF*. Photoprotective Acclimation of the Arabidopsis thaliana Leaf Proteome to Fluctuating Light. Front. Genet. 11:154.  
  3. Soh WT#, Demir F#, Dall E, Perrar A, Dahms SO, Kuppusamy M, Brandstetter H, Huesgen PF*. ExteNDing Proteome Coverage with Legumain as a Highly Specific Digestion Protease. Anal Chem. 92(4):2961-2971. 

2019

  1. Weng SSH,# Demir F#, Ergin EK, Dirnberger S, Uzozie A, Tuscher D, Nierves L, Tsui J, Huesgen PF*, Lange PF*. Sensitive Determination of Proteolytic Proteoforms in Limited Microscale Proteome Samples. Mol Cell Proteomics 18(11):2335-2347.
  2. Niedermaier S, Huesgen PF*. Positional proteomics for identification of secreted proteoforms released by site-specific processing of membrane proteins. Biochim Biophys Acta Proteins Proteom 1867(12):140138. Review.
  3. Huber CV, Jakobs BD#, Mishra LS#, Niedermaier S#, Stift M, Winter G, Adamska I, Funk C*, Huesgen PF*, Funck D*. DEG10 contributes to mitochondrial proteostasis, root growth, and seed yield in Arabidopsis. J Exp Bot 70(19):5423-5436.
  4. Misas Villamil JC, Mueller AN, Demir F, Meyer U, Ökmen B, Schulze Hüynck J, Breuer M, Dauben H, Win J, Huesgen PF, Doehlemann G. A fungal substrate mimicking molecule suppresses plant immunity via an inter-kingdom conserved motif. Nat Commun 10(1):1576. 
  5. Krichel C, Möckel C, Schillinger O, Huesgen PF, Sticht H, Strodel B, Weiergräber OH, Willbold D, Neudecker P. Solution structure of the autophagy-related protein LC3C reveals a polyproline II motif on a mobile tether with phosphorylation site. Sci Rep 9(1):14167.
  6. Zelanis A, Oliveira AK, Prudova A, Huesgen PF, Tashima AK, Kizhakkedathu J, Overall CM, Serrano SMT. Deep Profiling of the Cleavage Specificity and Human Substrates of Snake Venom Metalloprotease HF3 by Proteomic Identification of Cleavage Site Specificity (PICS) Using Proteome Derived Peptide Libraries and Terminal Amine Isotopic Labeling of Substrates (TAILS) N-Terminomics. J Proteome Res 18(9):3419-3428. 
  7. Drescher HK, Weiskirchen R, Fülöp A, Hopf C, González de San Román E, Huesgen PF, de Bruin A, Bongiovanni L, Christ A, Tolba R, Trautwein C, Kroy DC. The Influence of Different Fat Sources on Steatohepatitis and Fibrosis Development in the Western Diet Mouse Model of Non-alcoholic Steatohepatitis (NASH). Front Physiol 10:770.
  8. Elfgen A, Hupert M, Bochinsky K, Tusche M, González de San Román Martin E, Gering I, Sacchi S, Pollegioni L, Huesgen PF, Hartmann R, Santiago-Schübel B, Kutzsche J, Willbold D. Metabolic resistance of the D-peptide RD2 developed for direct elimination of amyloid-β oligomers. Sci Rep 9(1):5715. 
  9. Perrar A, Dissmeyer N*, Huesgen PF*. New beginnings and new ends – Methods for large-scale characterization of protein termini and their use in plant biology. J Exp Bot 70(7):2021-2038.
  10. Soares A, Niedermaier S, Faro R, Loos A, Manadas B, Faro C, Huesgen PF*, Cheung AY*, Simões I*. An atypical aspartic protease modulates lateral root development in Arabidopsis thaliana. J Exp Bot 70(7):2157-2171. 
  11. Demir F*, Perrar A*, Huesgen PF*. Das maßgeschneiderte Proteom: Proteinmodifikation durch Proteolyse. BIOspektrum 25:41-44. Review.
  12. Dahms SO, Demir F, Huesgen PF, Thorn K, Brandstetter H. Sirtilins - the new old members of the vitamin K-dependent coagulation factor family. J Thromb Haemost 17:470-481.
  13. Späth MR, Bartram MP, Palacio-Escat N, Hoyer KJR, Debes C, Demir F, Schroeter CB, Mandel AM, Grundmann F, Ciarimboli G, Beyer A, Kizhakkedathu JN, Brodesser S, Göbel H, Becker JU, Benzing T, Schermer B, Höhne M, Burst V, Saez-Rodriguez J, Huesgen PF, Müller RU, Rinschen MM. The proteome microenvironment determines the protective effect of preconditioning in cisplatin-induced acute kidney injury. Kidney Int 95:333-349. 

2018

  1. Lange J, Demir F, Huesgen PF, Baumann U, von Elert E, Pichlo C. Heterologous expression and characterization of a novel serine protease from Daphnia magna: A possible role in susceptibility to toxic cyanobacteria. Aquat Toxicol, 205:140-7.​
  2. Rinschen MR, Huesgen PF, Koch R. The Podocyte Protease Web: Uncovering the Gatekeepers of Glomerular Disease. Am J Physiol Renal Physiol 315:F1812-16. Review.
  3. Ökmen B, Kemmerich B, Hilbig D, Wemhöner R, Aschenbroich J, Perrar A, Huesgen PF, Schipper K, Doehlemann G. Dual function of a secreted fungalysin metalloprotease in Ustilago maydis. New Phytol 220:249-61.
  4. González de San Román E#, Bidmon HJ#, Malisic M, Susnea I, Küppers A, Hübbers R, Wree A, Nischwitz V, Amunts K*, Huesgen PF*. Molecular composition of the human primary visual cortex profiled by multimodal mass spectrometry imaging. Brain Struct Funct 223:2767-2783.
  5. Demir F#, Niedermaier S#, Villamor JG, Huesgen PF*. Quantitative proteomics in plant protease substrate identification. New Phytol 218(3):936-943. Review.

2017

  1. Rinschen MM*, Hoppe AK, Grahammer F, Kann M, Völker LA, Schurek EM, Binz J, Höhne M, Demir F, Malisic M, Huber TB, Kurschat C, Kizhakkedathu JN, Schermer B, Huesgen PF*, Benzing T*. N-Degradomic analysis reveals a proteolytic network processing the podocyte cytoskeleton. J Am Soc Nephrol, 28: 2867-2878. Highlight comment in Nat Rev Nephrol 13(10):596. 
  2. Demir F, Niedermaier S, Kizhakkedathu JN, Huesgen PF*. Profiling of Protein N-Termini and Their Modifications in Complex Samples. Methods Mol Biol, 574:35-50. 

2016

  1. Biniossek ML, Niemer M, Maksimchuk K, Mayer B, Fuchs J, Huesgen PF, McCafferty DG, Turk B, Fritz G, Mayer J, Haecker G, Mach L, Schilling O. Identification of Protease Specificity by Combining Proteome-Derived Peptide Libraries and Quantitative Proteomics. Mol Cell Proteomics, 15:2515-24. 
  2. Leal AR, Cruz R, Bur D, Huesgen PF, Faro R, Manadas B, Wlodawer A, Faro C,  Simões I. Enzymatic properties, evidence for in vivo expression, and intracellular localization of shewasin D, the pepsin homolog from Shewanella denitrificans. Sci Rep 6:23869. 
  3. Eckhard U#, Huesgen PF#, Schilling O#, Bellac CL, Butler GS, Cox JH, Dufour A, Goebeler V, Kappelhoff R, auf dem Keller U, Klein T, Lange PF, Marino G, Morrison CJ, Prudova A, Rodriguez D, Starr AE, Wang Y, Overall CM. Active site specificity profiling datasets of matrix metalloproteinases (MMP) 1, 2, 3, 7, 8, 9, 12, 13, 14. Data Brief 7:299-310. 
  4. Eckhard U#, Huesgen PF#, Schilling O#, Bellac CL, Butler GS, Cox JH, Dufour A, Goebeler V, Kappelhoff R, auf dem Keller U, Klein T, Lange PF, Marino G, Morrison CJ, Prudova A, Rodriguez D, Starr AE, Wang Y, Overall CM. Active Site Specificity of the Matrix Metalloproteinase Family: Proteomic Identification of 4,300 Cleavage Sites by Nine MMPs explored with Structural and Synthetic Peptide Cleavage Analyses. Matrix Biol 49:37-90. 

2015

  1. Zelanis A, Huesgen PF, Oliveira AK, Tashima AK, Serrano SM and Overall CM. Snake venom serine proteinases specificity mapping by proteomic identification of cleavage sites. J Proteomics 113:260-7. 
  2. Huesgen PF, Lange PF, Rogers LD, Solis N, Eckhard U, Kleifeld O, Goulas T, Gomis-Rüth FX and Overall CM. LysargiNase mirrors trypsin for protein C-terminal and methylation-site identification. Nat Methods 12:55-8. 7